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cas9 padh99 plasmid  (Addgene inc)


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    Structured Review

    Addgene inc cas9 padh99 plasmid
    A . amplification of Fragment A by PCR; B . amplification of Fragment B containing the gRNA by PCR; C . amplification of Fragment C by fusion PCR; D . digestion of the <t>Cas9</t> plasmid using the MssI restriction enzyme to generate the final Cas9 cassette; E . amplification of the dDNA cassette (GFP) by PCR; F . integration of the Cas9 cassette and Fragment C in the C. albicans HIS1 locus by homologous recombination.
    Cas9 Padh99 Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cas9+padh99+plasmid/bio_rxiv__2025__05__31__657172-101-14-21?v=Addgene+inc
    Average 93 stars, based on 2 article reviews
    cas9 padh99 plasmid - by Bioz Stars, 2026-08
    93/100 stars

    Images

    1) Product Images from "dnaudit + pydnaweb: A lightweight text-based planning and documentation workflow for genetic cloning with automatic verification"

    Article Title: dnaudit + pydnaweb: A lightweight text-based planning and documentation workflow for genetic cloning with automatic verification

    Journal: bioRxiv

    doi: 10.1101/2025.05.31.657172

    A . amplification of Fragment A by PCR; B . amplification of Fragment B containing the gRNA by PCR; C . amplification of Fragment C by fusion PCR; D . digestion of the Cas9 plasmid using the MssI restriction enzyme to generate the final Cas9 cassette; E . amplification of the dDNA cassette (GFP) by PCR; F . integration of the Cas9 cassette and Fragment C in the C. albicans HIS1 locus by homologous recombination.
    Figure Legend Snippet: A . amplification of Fragment A by PCR; B . amplification of Fragment B containing the gRNA by PCR; C . amplification of Fragment C by fusion PCR; D . digestion of the Cas9 plasmid using the MssI restriction enzyme to generate the final Cas9 cassette; E . amplification of the dDNA cassette (GFP) by PCR; F . integration of the Cas9 cassette and Fragment C in the C. albicans HIS1 locus by homologous recombination.

    Techniques Used: Amplification, Plasmid Preparation, Homologous Recombination

    The Cas9 endonuclease is guided by the gRNA to the target site within the ATO1 gene. The gRNA binds to the complementary DNA sequence, allowing Cas9 to introduce a double-strand break (DSB) at that locus (Figure S2). The donor DNA cassette containing the gene encoding GFP facilitates homology-directed repair (HDR), resulting in the insertion of the GFP sequence into the 3’ end of the ATO1 gene.
    Figure Legend Snippet: The Cas9 endonuclease is guided by the gRNA to the target site within the ATO1 gene. The gRNA binds to the complementary DNA sequence, allowing Cas9 to introduce a double-strand break (DSB) at that locus (Figure S2). The donor DNA cassette containing the gene encoding GFP facilitates homology-directed repair (HDR), resulting in the insertion of the GFP sequence into the 3’ end of the ATO1 gene.

    Techniques Used: Sequencing, Introduce



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    cas9 padh99 plasmid  (Addgene inc)
    93
    Addgene inc cas9 padh99 plasmid
    A . amplification of Fragment A by PCR; B . amplification of Fragment B containing the gRNA by PCR; C . amplification of Fragment C by fusion PCR; D . digestion of the <t>Cas9</t> plasmid using the MssI restriction enzyme to generate the final Cas9 cassette; E . amplification of the dDNA cassette (GFP) by PCR; F . integration of the Cas9 cassette and Fragment C in the C. albicans HIS1 locus by homologous recombination.
    Cas9 Padh99 Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cas9+padh99+plasmid/bio_rxiv__2025__05__31__657172-101-14-21?v=Addgene+inc
    Average 93 stars, based on 1 article reviews
    cas9 padh99 plasmid - by Bioz Stars, 2026-08
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    A . amplification of Fragment A by PCR; B . amplification of Fragment B containing the gRNA by PCR; C . amplification of Fragment C by fusion PCR; D . digestion of the Cas9 plasmid using the MssI restriction enzyme to generate the final Cas9 cassette; E . amplification of the dDNA cassette (GFP) by PCR; F . integration of the Cas9 cassette and Fragment C in the C. albicans HIS1 locus by homologous recombination.

    Journal: bioRxiv

    Article Title: dnaudit + pydnaweb: A lightweight text-based planning and documentation workflow for genetic cloning with automatic verification

    doi: 10.1101/2025.05.31.657172

    Figure Lengend Snippet: A . amplification of Fragment A by PCR; B . amplification of Fragment B containing the gRNA by PCR; C . amplification of Fragment C by fusion PCR; D . digestion of the Cas9 plasmid using the MssI restriction enzyme to generate the final Cas9 cassette; E . amplification of the dDNA cassette (GFP) by PCR; F . integration of the Cas9 cassette and Fragment C in the C. albicans HIS1 locus by homologous recombination.

    Article Snippet: Finally, Fragment C was co-transformed into C. albicans SC5314 together with the appropriately digested CAS9 pADH99 plasmid (with MssI restriction enzyme; Addgene plasmid # 90979; ) and the donor DNA. dDNAs ( ) were amplified from the template CIp10–γmGFP (Addgene plasmid # 163119, 27) using the oligonucleotides dDNA_ATO1_GFP_Fw and dDNA_ATO1_GFP_Rv as forward and reverse primers, respectively ( Table S1 ).

    Techniques: Amplification, Plasmid Preparation, Homologous Recombination

    The Cas9 endonuclease is guided by the gRNA to the target site within the ATO1 gene. The gRNA binds to the complementary DNA sequence, allowing Cas9 to introduce a double-strand break (DSB) at that locus (Figure S2). The donor DNA cassette containing the gene encoding GFP facilitates homology-directed repair (HDR), resulting in the insertion of the GFP sequence into the 3’ end of the ATO1 gene.

    Journal: bioRxiv

    Article Title: dnaudit + pydnaweb: A lightweight text-based planning and documentation workflow for genetic cloning with automatic verification

    doi: 10.1101/2025.05.31.657172

    Figure Lengend Snippet: The Cas9 endonuclease is guided by the gRNA to the target site within the ATO1 gene. The gRNA binds to the complementary DNA sequence, allowing Cas9 to introduce a double-strand break (DSB) at that locus (Figure S2). The donor DNA cassette containing the gene encoding GFP facilitates homology-directed repair (HDR), resulting in the insertion of the GFP sequence into the 3’ end of the ATO1 gene.

    Article Snippet: Finally, Fragment C was co-transformed into C. albicans SC5314 together with the appropriately digested CAS9 pADH99 plasmid (with MssI restriction enzyme; Addgene plasmid # 90979; ) and the donor DNA. dDNAs ( ) were amplified from the template CIp10–γmGFP (Addgene plasmid # 163119, 27) using the oligonucleotides dDNA_ATO1_GFP_Fw and dDNA_ATO1_GFP_Rv as forward and reverse primers, respectively ( Table S1 ).

    Techniques: Sequencing, Introduce